Fig 2: Gene ontology (GO) enrichment analysis for the top 50 genes positively correlated with SPDL1 in CRC. The three GO categories [cellular component (CC), biological process (BP) and molecular function MF)] were detected using DAVID Bioinformatics Tool. The top 15 enriched terms for BP (A) and CC (C), as well as 10 terms for MF (E) are displayed. (A,C,E) The horizontal axis of bar chart represents the –log10 (p-value) for the GO terms represented on the vertical axis. (B,D,F) Heatmaps based on the target genes.

Fig 3: Kaplan-Meier survival curves and log-rank test for overall survival of CRC patients based on (A) SPDL1 protein expression and (B) SPDL1 mRNA expression.

Fig 4: (A) The expression level of SPDL1 mRNA in colorectal cancer compared to normal tissues. Analysis was performed based on gene expression data sourced from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. The error bars present the range from minimum to maximum values of data. Asterisks (*) indicate statistically significant differences (p < 0.05). (B) The optimal cut-off value for SPDL1 mRNA expression level was established using Evaluate Cutpoints software.

Fig 5: (A) Immunohistochemical expression of SPDL1 in colorectal cancer compared to non-cancerous adjacent tissues. The error bars present the range from minimum to maximum values of data. Asterisk (*) indicates statistically significant difference (p < 0.05). (B) The optimal cut-off value established for SPDL1 based on IRS scores and Evaluate Cutpoints software.